bacillus subtilis Search Results


japan collection  (ATCC)
94
ATCC japan collection
Japan Collection, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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organisms b  (ATCC)
90
ATCC organisms b
Organisms B, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ipi  (ATCC)
93
ATCC ipi
Ipi, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna  (ATCC)
96
ATCC dna
FIG. 3. Survey of antibacterially acting metabolites through bioautography. Supernatants prepared from strain FZB 42 and mutant strains (A, C) and B. subtilis <t>JH642</t> <t>(sfp0)</t> and OKB105 (sfp) (B, D) grown for 12 h in Landy medium (24) were separated by TLC and sandwiched with indicator strains E. carotovora (top) and B. megaterium (bottom) as described in Materials and Methods. Inhibition zones with no or reduced growth are indicative of antibiotic activities. Abbreviations: B, bacillaene synthesized by gene cluster pks1 (bae); S, surfactin synthesized by the srf gene cluster (20); PK2, unknown polyketide synthesized by gene cluster pks2; D, difficidin or oxydifficidin synthesized by gene cluster pks3 (dif); X, unknown metabolite with antibacterial activity not dependent on phosphopantetheine transfer catalyzed by the sfp gene product. The weak antibacterial activity exerted by gene cluster pks2 is indicated by circles. Strain CH1 (srfAA::ermAM) is deficient in surfactin synthesis (20). Strain CH2 (fen::Cmr srfAA::ermAM) was obtained by transformation of strain AK2 (20) with chromosomal <t>DNA</t> isolated from strain CH1. Strain CH4 harbors a knockout mutation of the yczE gene (23) and will be described elsewhere. The other mutant strains used for bioautography were CH3 (sfp::ermAM), CH6 (pks1KS1::cat), CH7 (pks2KS1::cat), CH8 (pks3KS1::ermAM), CH11 (pks1KS1::cat pks3KS1::ermAM), CH12 (pks1KS1::cat pks3KS1::ermAM), JH (JH642 pheA trpC2 sfp0), and OKB (OKB105 pheA sfp0).
Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b amyloliquefaciens atcc 8473  (ATCC)
90
ATCC b amyloliquefaciens atcc 8473
FIG. 3. Survey of antibacterially acting metabolites through bioautography. Supernatants prepared from strain FZB 42 and mutant strains (A, C) and B. subtilis <t>JH642</t> <t>(sfp0)</t> and OKB105 (sfp) (B, D) grown for 12 h in Landy medium (24) were separated by TLC and sandwiched with indicator strains E. carotovora (top) and B. megaterium (bottom) as described in Materials and Methods. Inhibition zones with no or reduced growth are indicative of antibiotic activities. Abbreviations: B, bacillaene synthesized by gene cluster pks1 (bae); S, surfactin synthesized by the srf gene cluster (20); PK2, unknown polyketide synthesized by gene cluster pks2; D, difficidin or oxydifficidin synthesized by gene cluster pks3 (dif); X, unknown metabolite with antibacterial activity not dependent on phosphopantetheine transfer catalyzed by the sfp gene product. The weak antibacterial activity exerted by gene cluster pks2 is indicated by circles. Strain CH1 (srfAA::ermAM) is deficient in surfactin synthesis (20). Strain CH2 (fen::Cmr srfAA::ermAM) was obtained by transformation of strain AK2 (20) with chromosomal <t>DNA</t> isolated from strain CH1. Strain CH4 harbors a knockout mutation of the yczE gene (23) and will be described elsewhere. The other mutant strains used for bioautography were CH3 (sfp::ermAM), CH6 (pks1KS1::cat), CH7 (pks2KS1::cat), CH8 (pks3KS1::ermAM), CH11 (pks1KS1::cat pks3KS1::ermAM), CH12 (pks1KS1::cat pks3KS1::ermAM), JH (JH642 pheA trpC2 sfp0), and OKB (OKB105 pheA sfp0).
B Amyloliquefaciens Atcc 8473, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli um2 psw186 expressing b subtilis atcc 31954 tm perhydrolase  (ATCC)
95
ATCC e coli um2 psw186 expressing b subtilis atcc 31954 tm perhydrolase
FIG. 3. Survey of antibacterially acting metabolites through bioautography. Supernatants prepared from strain FZB 42 and mutant strains (A, C) and B. subtilis <t>JH642</t> <t>(sfp0)</t> and OKB105 (sfp) (B, D) grown for 12 h in Landy medium (24) were separated by TLC and sandwiched with indicator strains E. carotovora (top) and B. megaterium (bottom) as described in Materials and Methods. Inhibition zones with no or reduced growth are indicative of antibiotic activities. Abbreviations: B, bacillaene synthesized by gene cluster pks1 (bae); S, surfactin synthesized by the srf gene cluster (20); PK2, unknown polyketide synthesized by gene cluster pks2; D, difficidin or oxydifficidin synthesized by gene cluster pks3 (dif); X, unknown metabolite with antibacterial activity not dependent on phosphopantetheine transfer catalyzed by the sfp gene product. The weak antibacterial activity exerted by gene cluster pks2 is indicated by circles. Strain CH1 (srfAA::ermAM) is deficient in surfactin synthesis (20). Strain CH2 (fen::Cmr srfAA::ermAM) was obtained by transformation of strain AK2 (20) with chromosomal <t>DNA</t> isolated from strain CH1. Strain CH4 harbors a knockout mutation of the yczE gene (23) and will be described elsewhere. The other mutant strains used for bioautography were CH3 (sfp::ermAM), CH6 (pks1KS1::cat), CH7 (pks2KS1::cat), CH8 (pks3KS1::ermAM), CH11 (pks1KS1::cat pks3KS1::ermAM), CH12 (pks1KS1::cat pks3KS1::ermAM), JH (JH642 pheA trpC2 sfp0), and OKB (OKB105 pheA sfp0).
E Coli Um2 Psw186 Expressing B Subtilis Atcc 31954 Tm Perhydrolase, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atcc 31098  (ATCC)
90
ATCC atcc 31098
FIG. 3. Survey of antibacterially acting metabolites through bioautography. Supernatants prepared from strain FZB 42 and mutant strains (A, C) and B. subtilis <t>JH642</t> <t>(sfp0)</t> and OKB105 (sfp) (B, D) grown for 12 h in Landy medium (24) were separated by TLC and sandwiched with indicator strains E. carotovora (top) and B. megaterium (bottom) as described in Materials and Methods. Inhibition zones with no or reduced growth are indicative of antibiotic activities. Abbreviations: B, bacillaene synthesized by gene cluster pks1 (bae); S, surfactin synthesized by the srf gene cluster (20); PK2, unknown polyketide synthesized by gene cluster pks2; D, difficidin or oxydifficidin synthesized by gene cluster pks3 (dif); X, unknown metabolite with antibacterial activity not dependent on phosphopantetheine transfer catalyzed by the sfp gene product. The weak antibacterial activity exerted by gene cluster pks2 is indicated by circles. Strain CH1 (srfAA::ermAM) is deficient in surfactin synthesis (20). Strain CH2 (fen::Cmr srfAA::ermAM) was obtained by transformation of strain AK2 (20) with chromosomal <t>DNA</t> isolated from strain CH1. Strain CH4 harbors a knockout mutation of the yczE gene (23) and will be described elsewhere. The other mutant strains used for bioautography were CH3 (sfp::ermAM), CH6 (pks1KS1::cat), CH7 (pks2KS1::cat), CH8 (pks3KS1::ermAM), CH11 (pks1KS1::cat pks3KS1::ermAM), CH12 (pks1KS1::cat pks3KS1::ermAM), JH (JH642 pheA trpC2 sfp0), and OKB (OKB105 pheA sfp0).
Atcc 31098, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacillus velezensis atcc pta 6737  (ATCC)
94
ATCC bacillus velezensis atcc pta 6737
FIG. 3. Survey of antibacterially acting metabolites through bioautography. Supernatants prepared from strain FZB 42 and mutant strains (A, C) and B. subtilis <t>JH642</t> <t>(sfp0)</t> and OKB105 (sfp) (B, D) grown for 12 h in Landy medium (24) were separated by TLC and sandwiched with indicator strains E. carotovora (top) and B. megaterium (bottom) as described in Materials and Methods. Inhibition zones with no or reduced growth are indicative of antibiotic activities. Abbreviations: B, bacillaene synthesized by gene cluster pks1 (bae); S, surfactin synthesized by the srf gene cluster (20); PK2, unknown polyketide synthesized by gene cluster pks2; D, difficidin or oxydifficidin synthesized by gene cluster pks3 (dif); X, unknown metabolite with antibacterial activity not dependent on phosphopantetheine transfer catalyzed by the sfp gene product. The weak antibacterial activity exerted by gene cluster pks2 is indicated by circles. Strain CH1 (srfAA::ermAM) is deficient in surfactin synthesis (20). Strain CH2 (fen::Cmr srfAA::ermAM) was obtained by transformation of strain AK2 (20) with chromosomal <t>DNA</t> isolated from strain CH1. Strain CH4 harbors a knockout mutation of the yczE gene (23) and will be described elsewhere. The other mutant strains used for bioautography were CH3 (sfp::ermAM), CH6 (pks1KS1::cat), CH7 (pks2KS1::cat), CH8 (pks3KS1::ermAM), CH11 (pks1KS1::cat pks3KS1::ermAM), CH12 (pks1KS1::cat pks3KS1::ermAM), JH (JH642 pheA trpC2 sfp0), and OKB (OKB105 pheA sfp0).
Bacillus Velezensis Atcc Pta 6737, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacillus subtilis atcc 6337  (ATCC)
92
ATCC bacillus subtilis atcc 6337
FIG. 3. Survey of antibacterially acting metabolites through bioautography. Supernatants prepared from strain FZB 42 and mutant strains (A, C) and B. subtilis <t>JH642</t> <t>(sfp0)</t> and OKB105 (sfp) (B, D) grown for 12 h in Landy medium (24) were separated by TLC and sandwiched with indicator strains E. carotovora (top) and B. megaterium (bottom) as described in Materials and Methods. Inhibition zones with no or reduced growth are indicative of antibiotic activities. Abbreviations: B, bacillaene synthesized by gene cluster pks1 (bae); S, surfactin synthesized by the srf gene cluster (20); PK2, unknown polyketide synthesized by gene cluster pks2; D, difficidin or oxydifficidin synthesized by gene cluster pks3 (dif); X, unknown metabolite with antibacterial activity not dependent on phosphopantetheine transfer catalyzed by the sfp gene product. The weak antibacterial activity exerted by gene cluster pks2 is indicated by circles. Strain CH1 (srfAA::ermAM) is deficient in surfactin synthesis (20). Strain CH2 (fen::Cmr srfAA::ermAM) was obtained by transformation of strain AK2 (20) with chromosomal <t>DNA</t> isolated from strain CH1. Strain CH4 harbors a knockout mutation of the yczE gene (23) and will be described elsewhere. The other mutant strains used for bioautography were CH3 (sfp::ermAM), CH6 (pks1KS1::cat), CH7 (pks2KS1::cat), CH8 (pks3KS1::ermAM), CH11 (pks1KS1::cat pks3KS1::ermAM), CH12 (pks1KS1::cat pks3KS1::ermAM), JH (JH642 pheA trpC2 sfp0), and OKB (OKB105 pheA sfp0).
Bacillus Subtilis Atcc 6337, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacillus subtilis  (ATCC)
90
ATCC bacillus subtilis
FIG. 3. Survey of antibacterially acting metabolites through bioautography. Supernatants prepared from strain FZB 42 and mutant strains (A, C) and B. subtilis <t>JH642</t> <t>(sfp0)</t> and OKB105 (sfp) (B, D) grown for 12 h in Landy medium (24) were separated by TLC and sandwiched with indicator strains E. carotovora (top) and B. megaterium (bottom) as described in Materials and Methods. Inhibition zones with no or reduced growth are indicative of antibiotic activities. Abbreviations: B, bacillaene synthesized by gene cluster pks1 (bae); S, surfactin synthesized by the srf gene cluster (20); PK2, unknown polyketide synthesized by gene cluster pks2; D, difficidin or oxydifficidin synthesized by gene cluster pks3 (dif); X, unknown metabolite with antibacterial activity not dependent on phosphopantetheine transfer catalyzed by the sfp gene product. The weak antibacterial activity exerted by gene cluster pks2 is indicated by circles. Strain CH1 (srfAA::ermAM) is deficient in surfactin synthesis (20). Strain CH2 (fen::Cmr srfAA::ermAM) was obtained by transformation of strain AK2 (20) with chromosomal <t>DNA</t> isolated from strain CH1. Strain CH4 harbors a knockout mutation of the yczE gene (23) and will be described elsewhere. The other mutant strains used for bioautography were CH3 (sfp::ermAM), CH6 (pks1KS1::cat), CH7 (pks2KS1::cat), CH8 (pks3KS1::ermAM), CH11 (pks1KS1::cat pks3KS1::ermAM), CH12 (pks1KS1::cat pks3KS1::ermAM), JH (JH642 pheA trpC2 sfp0), and OKB (OKB105 pheA sfp0).
Bacillus Subtilis, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gallibacterium anatis ccug15563t  (ATCC)
86
ATCC gallibacterium anatis ccug15563t
FIG. 3. Survey of antibacterially acting metabolites through bioautography. Supernatants prepared from strain FZB 42 and mutant strains (A, C) and B. subtilis <t>JH642</t> <t>(sfp0)</t> and OKB105 (sfp) (B, D) grown for 12 h in Landy medium (24) were separated by TLC and sandwiched with indicator strains E. carotovora (top) and B. megaterium (bottom) as described in Materials and Methods. Inhibition zones with no or reduced growth are indicative of antibiotic activities. Abbreviations: B, bacillaene synthesized by gene cluster pks1 (bae); S, surfactin synthesized by the srf gene cluster (20); PK2, unknown polyketide synthesized by gene cluster pks2; D, difficidin or oxydifficidin synthesized by gene cluster pks3 (dif); X, unknown metabolite with antibacterial activity not dependent on phosphopantetheine transfer catalyzed by the sfp gene product. The weak antibacterial activity exerted by gene cluster pks2 is indicated by circles. Strain CH1 (srfAA::ermAM) is deficient in surfactin synthesis (20). Strain CH2 (fen::Cmr srfAA::ermAM) was obtained by transformation of strain AK2 (20) with chromosomal <t>DNA</t> isolated from strain CH1. Strain CH4 harbors a knockout mutation of the yczE gene (23) and will be described elsewhere. The other mutant strains used for bioautography were CH3 (sfp::ermAM), CH6 (pks1KS1::cat), CH7 (pks2KS1::cat), CH8 (pks3KS1::ermAM), CH11 (pks1KS1::cat pks3KS1::ermAM), CH12 (pks1KS1::cat pks3KS1::ermAM), JH (JH642 pheA trpC2 sfp0), and OKB (OKB105 pheA sfp0).
Gallibacterium Anatis Ccug15563t, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rsv b wv  (ATCC)
98
ATCC rsv b wv
FIG. 3. Survey of antibacterially acting metabolites through bioautography. Supernatants prepared from strain FZB 42 and mutant strains (A, C) and B. subtilis <t>JH642</t> <t>(sfp0)</t> and OKB105 (sfp) (B, D) grown for 12 h in Landy medium (24) were separated by TLC and sandwiched with indicator strains E. carotovora (top) and B. megaterium (bottom) as described in Materials and Methods. Inhibition zones with no or reduced growth are indicative of antibiotic activities. Abbreviations: B, bacillaene synthesized by gene cluster pks1 (bae); S, surfactin synthesized by the srf gene cluster (20); PK2, unknown polyketide synthesized by gene cluster pks2; D, difficidin or oxydifficidin synthesized by gene cluster pks3 (dif); X, unknown metabolite with antibacterial activity not dependent on phosphopantetheine transfer catalyzed by the sfp gene product. The weak antibacterial activity exerted by gene cluster pks2 is indicated by circles. Strain CH1 (srfAA::ermAM) is deficient in surfactin synthesis (20). Strain CH2 (fen::Cmr srfAA::ermAM) was obtained by transformation of strain AK2 (20) with chromosomal <t>DNA</t> isolated from strain CH1. Strain CH4 harbors a knockout mutation of the yczE gene (23) and will be described elsewhere. The other mutant strains used for bioautography were CH3 (sfp::ermAM), CH6 (pks1KS1::cat), CH7 (pks2KS1::cat), CH8 (pks3KS1::ermAM), CH11 (pks1KS1::cat pks3KS1::ermAM), CH12 (pks1KS1::cat pks3KS1::ermAM), JH (JH642 pheA trpC2 sfp0), and OKB (OKB105 pheA sfp0).
Rsv B Wv, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 3. Survey of antibacterially acting metabolites through bioautography. Supernatants prepared from strain FZB 42 and mutant strains (A, C) and B. subtilis JH642 (sfp0) and OKB105 (sfp) (B, D) grown for 12 h in Landy medium (24) were separated by TLC and sandwiched with indicator strains E. carotovora (top) and B. megaterium (bottom) as described in Materials and Methods. Inhibition zones with no or reduced growth are indicative of antibiotic activities. Abbreviations: B, bacillaene synthesized by gene cluster pks1 (bae); S, surfactin synthesized by the srf gene cluster (20); PK2, unknown polyketide synthesized by gene cluster pks2; D, difficidin or oxydifficidin synthesized by gene cluster pks3 (dif); X, unknown metabolite with antibacterial activity not dependent on phosphopantetheine transfer catalyzed by the sfp gene product. The weak antibacterial activity exerted by gene cluster pks2 is indicated by circles. Strain CH1 (srfAA::ermAM) is deficient in surfactin synthesis (20). Strain CH2 (fen::Cmr srfAA::ermAM) was obtained by transformation of strain AK2 (20) with chromosomal DNA isolated from strain CH1. Strain CH4 harbors a knockout mutation of the yczE gene (23) and will be described elsewhere. The other mutant strains used for bioautography were CH3 (sfp::ermAM), CH6 (pks1KS1::cat), CH7 (pks2KS1::cat), CH8 (pks3KS1::ermAM), CH11 (pks1KS1::cat pks3KS1::ermAM), CH12 (pks1KS1::cat pks3KS1::ermAM), JH (JH642 pheA trpC2 sfp0), and OKB (OKB105 pheA sfp0).

Journal: Journal of Bacteriology

Article Title: Structural and Functional Characterization of Three Polyketide Synthase Gene Clusters in Bacillus amyloliquefaciens FZB 42

doi: 10.1128/jb.00052-06

Figure Lengend Snippet: FIG. 3. Survey of antibacterially acting metabolites through bioautography. Supernatants prepared from strain FZB 42 and mutant strains (A, C) and B. subtilis JH642 (sfp0) and OKB105 (sfp) (B, D) grown for 12 h in Landy medium (24) were separated by TLC and sandwiched with indicator strains E. carotovora (top) and B. megaterium (bottom) as described in Materials and Methods. Inhibition zones with no or reduced growth are indicative of antibiotic activities. Abbreviations: B, bacillaene synthesized by gene cluster pks1 (bae); S, surfactin synthesized by the srf gene cluster (20); PK2, unknown polyketide synthesized by gene cluster pks2; D, difficidin or oxydifficidin synthesized by gene cluster pks3 (dif); X, unknown metabolite with antibacterial activity not dependent on phosphopantetheine transfer catalyzed by the sfp gene product. The weak antibacterial activity exerted by gene cluster pks2 is indicated by circles. Strain CH1 (srfAA::ermAM) is deficient in surfactin synthesis (20). Strain CH2 (fen::Cmr srfAA::ermAM) was obtained by transformation of strain AK2 (20) with chromosomal DNA isolated from strain CH1. Strain CH4 harbors a knockout mutation of the yczE gene (23) and will be described elsewhere. The other mutant strains used for bioautography were CH3 (sfp::ermAM), CH6 (pks1KS1::cat), CH7 (pks2KS1::cat), CH8 (pks3KS1::ermAM), CH11 (pks1KS1::cat pks3KS1::ermAM), CH12 (pks1KS1::cat pks3KS1::ermAM), JH (JH642 pheA trpC2 sfp0), and OKB (OKB105 pheA sfp0).

Article Snippet: JH642 trpC2 pheA1 sfp0 27 OKB105 pheA1 sfp , surfactin producer, JH642 transformed with DNA of ATCC 21332 27 ATCC 39320 Wild type, difficidin producer 53

Techniques: Mutagenesis, Inhibition, Synthesized, Activity Assay, Transformation Assay, Isolation, Knock-Out